mouse anti chicken mhc β antibody  (Developmental Studies Hybridoma Bank)

Journal: Antioxidants

Article Title: 25-Hydroxycholecalciferol Improves Cardiac Metabolic Adaption, Mitochondrial Biogenetics, and Redox Status to Ameliorate Pathological Remodeling and Functional Failure in Obese Chickens

doi: 10.3390/antiox13111426

Figure Lengend Snippet: Effects of dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation on heart failure marker expressions of broiler breeder hens provided with restricted (R) or ad libitum (Ad) feed intake. Hearts collected at 28, 33, and 45 weeks were used for cardiac failure marker, BNP (brain natriuretic peptide), and MHC-β (myosin heavy chain, cardiac muscle beta) expressions through the qRT-PCR method with normalization to GADPH (n = 4 ( A )) and by immunohistochemistry (n = 3 ( B )). All results are expressed as ratios relative to R-hens at 28 or 33 weeks *; significant difference by Ad-feed intake (vs. corresponding R-hens, p < 0.05) +; significant difference by 25-OH-D3 (vs. R- or Ad-hens, p < 0.05).

Article Snippet: An antigen retrieval method as described previously was used for immunohistochemical analysis with a mouse anti-chicken MHC-β antibody (clone 2E9, Developmental Studies Hybridoma Bank, Iowa City, IA, USA) to visualize MHC-β expression and an avian-specific mouse monoclonal antibody conjugated with FITC (Clone KUL01, Abcam) for macrophage infiltration [ , ].

Techniques: Marker, Quantitative RT-PCR, Immunohistochemistry

Journal: Poultry Science

Article Title: Screening and selection of eubiotic compounds possessing immunomodulatory and anti- Clostridium perfringens properties

doi: 10.1016/j.psj.2024.103911

Figure Lengend Snippet: Expression of MHC-II surface expression by macrophages stimulated with eubiotic compounds. MQ-NCSU cells were treated with eubiotic compounds for 24 hrs. and cells were collected for staining with cell viability dye (Live/Dead near-IR) and anti-chicken MHC-II antibody. The stained cells were analyzed by flow cytometry, along with the staining control to gate on the fluorescent-positive cell populations. Panel A shows the representative histograms from each of the EC treatment groups depicting gates comprised of live MHC-II+ macrophage cell population. Panel B shows the group averages of live MHC-II+ cell frequencies in the indicated water-soluble and DMSO-soluble eubiotic compounds. Error bars represent mean value ± standard error. Asterisks above the group's averages plotted in the graph indicate statistical significance between the treatment and their corresponding solvent control groups: * P < 0.05.

Article Snippet: Primary antibody was added to each well (1 μg/10 6 cells) and stained for 30 mins on ice with mouse anti-chicken MHC-II β-chain antibody (clone 2G11) (Southern Biotech Inc., Birmingham, AL).

Techniques: Expressing, Staining, Flow Cytometry, Control, Solvent

Journal: Poultry Science

Article Title: Screening and selection of eubiotic compounds possessing immunomodulatory and anti- Clostridium perfringens properties

doi: 10.1016/j.psj.2024.103911

Figure Lengend Snippet: Summary of changes in the immune gene expression in MQ-NCSU macrophages treated with eubiotic compounds.

Article Snippet: Primary antibody was added to each well (1 μg/10 6 cells) and stained for 30 mins on ice with mouse anti-chicken MHC-II β-chain antibody (clone 2G11) (Southern Biotech Inc., Birmingham, AL).

Techniques: Gene Expression, Expressing